Journal: EMBO Reports

Article Title: T cell‐dependent bispecific antibodies alter organ‐specific endothelial cell–T cell interaction

doi: 10.15252/embr.202255532

Figure Lengend Snippet:

Article Snippet: TSA Cyanine 5 , Akoya Bioscience Inc. , NEL745001KT.

Techniques: Recombinant, Purification, Blocking Assay, Produced, RNAscope, Sequencing, Negative Control, Protease Inhibitor, Software, Multiplex Assay, Cell Isolation, Enzyme-linked Immunosorbent Assay, BIA-KA, Expressing

Journal: Cell reports

Article Title: Arid1a-Plagl1-Hh signaling is indispensable for differentiation-associated cell cycle arrest of tooth root progenitors

doi: 10.1016/j.celrep.2021.108964

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads; Cell Signaling Technology 9005), anti-Arid1a antibody (Cell Signaling, 12354), anti-Arid1b antibody (Cell Signaling, 92964), and rabbit IgG (Cell Signaling, 2729) were used following the manufacturer’s instructions.

Techniques: Recombinant, Protease Inhibitor, TUNEL Assay, In Situ, Imaging, RNAscope, Multiplex Assay, cDNA Synthesis, SYBR Green Assay, Chromatin Immunoprecipitation, Software

Journal: Neuron

Article Title: Anatomical barriers against SARS-CoV-2 neuroinvasion at vulnerable interfaces visualized in deceased COVID-19 patients

doi: 10.1016/j.neuron.2022.11.007

Figure Lengend Snippet: Infection of the respiratory mucosa with Delta, Omicron BA.1, or Omicron BA.2 Confocal images of sections through the respiratory mucosa of COVID #87 (Delta), #95 (Omicron BA.1), #104 (Omicron BA.1), #101 (Omicron BA.2), and #107 (Omicron BA.2) stained with RNAscope and IHC. Names of genes (italics) and proteins (roman) are at the bottom left, scale bars at the bottom right. DAPI served as nuclear stain. (A) N puncta are densely packed in cells harboring FOXJ1 puncta or containing KRT8-IR signal. (B) Nucleocapsid-IR signal is widespread. N-sense puncta and orf1ab-sense puncta reflect ongoing viral replication. (C) MUC5AC-IR mucus covers a stretch of respiratory epithelium with N-sense puncta in cells harboring FOXJ1 puncta. (D) S-sense puncta occur at a characteristic perinuclear position in nucleocapsid-IR cells. (E) Nucleocapsid-IR signal outlines the contours of ciliated cells, which harbor densely packed S puncta. (F) At the apical edge of a crypt or gland duct, KRT8-IR cells harbor N puncta. Deeper, clusters of extracellular N puncta reflect virions released from cells. (G) The dashed square in (F) imaged at higher magnification. (H) In a section near (G), extracellular S puncta colocalize with nucleocapsid-IR signal. (I and J) Omicron BA.2 (I) or Omicron BA.1 (J) swept through a broad swath of the respiratory epithelium, with nearly every nucleocapsid-IR cell harboring N-sense puncta and orf1ab-sense puncta.

Article Snippet: RNAscope Hydrogen Peroxide and Protease Plus , Advanced Cell Diagnostics , Cat# 322330.

Techniques: Infection, Staining, RNAscope, Data-independent acquisition

Journal: Neuron

Article Title: Anatomical barriers against SARS-CoV-2 neuroinvasion at vulnerable interfaces visualized in deceased COVID-19 patients

doi: 10.1016/j.neuron.2022.11.007

Figure Lengend Snippet:

Article Snippet: RNAscope Hydrogen Peroxide and Protease Plus , Advanced Cell Diagnostics , Cat# 322330.

Techniques: Marker, Recombinant, Control, RNAscope, Electron Microscopy, Amplification, Multiplex Assay, Software